Lab 2a:
-Introduction:
I know the picture isn't Eggs-actly professional, it was what I could manage. In this Egg-sparament, we place an Egg into a beaker with vinegar to see what happens to the Egg. We also work on mixing certain Materials together to see there effects and results! Egg-citing, I know, but I can't Spoil anything yet. Look at the rest of all the details below to see what happens next! Hope my puns weren't too Fowl. Okay, I am done. Sorry. I couldn't resist.
I know the picture isn't Eggs-actly professional, it was what I could manage. In this Egg-sparament, we place an Egg into a beaker with vinegar to see what happens to the Egg. We also work on mixing certain Materials together to see there effects and results! Egg-citing, I know, but I can't Spoil anything yet. Look at the rest of all the details below to see what happens next! Hope my puns weren't too Fowl. Okay, I am done. Sorry. I couldn't resist.
-Purpose:
1.) What are positive indicator tests for proteins, carbohydrates, and fats?
2.) Do parts of egg test positive for protein, carbohydrate, and/or fats?
~Materials:
-Eggs
-Beaker, 250 mL
-White vinegar
-Plastic wrap
-Slotted spoon
-Sodium chloride
-Distilled water
-Beaker, 100 mL
-Pipets, 5 mL
-Pipet pump, green
-Tubes, glass, 13x100 mm
-Peg racks for 13x100 mm tubes
-Glucose (dextrose)
-Benedict's Solution
-Hot plate stirrer
-Test tube holder
-Starch, soluble
-Lugol's Iodine Solution
-Vortex mixer
-Gelatin
-Sodium hydroxide
-Cupric sulfate 5-hydrate
-Oil
-Sudan IV Solution
-Scalpel handles, #4
-Scalpel blades, #22, for #4 handles
-Trays, plastic
~Part I: Separating Cell Structures
1.) Place an uncooked egg into a beaker of white vinegar. Label and cover it with plastic wrap. Leave it for 24 to 48 hours. While the shell dissolves, proceed to Part 2.
2.) Gently pick up the egg with a slotted spoon and rinse it in water to remove the vinegar after the 24 to 48 hour mark.
3.) Feel the outer membranes of the egg. Place the egg in a beaker of 5% NaCl solution for 24 hours to verify that the water can enter and leave the cell easily.
4.) After the 24 hours, gently rinse off the egg with water. Then place it into a beaker of distilled water for 24 hours to see what changes.
5.) Gently slice the egg's membranes open to let the egg white drip though the slots of the spoon into a 100-mL beaker. Then try to get all egg white into the beaker without damaging the yolk.
6.) Place the yolk of the egg into another breaker, with the egg membranes removed.
~Part II: Testing Standard Solutions
-Control so/ns +/-
-Know what is in standards
1.) What are positive indicator tests for proteins, carbohydrates, and fats?
2.) Do parts of egg test positive for protein, carbohydrate, and/or fats?
~Materials:
-Eggs
-Beaker, 250 mL
-White vinegar
-Plastic wrap
-Slotted spoon
-Sodium chloride
-Distilled water
-Beaker, 100 mL
-Pipets, 5 mL
-Pipet pump, green
-Tubes, glass, 13x100 mm
-Peg racks for 13x100 mm tubes
-Glucose (dextrose)
-Benedict's Solution
-Hot plate stirrer
-Test tube holder
-Starch, soluble
-Lugol's Iodine Solution
-Vortex mixer
-Gelatin
-Sodium hydroxide
-Cupric sulfate 5-hydrate
-Oil
-Sudan IV Solution
-Scalpel handles, #4
-Scalpel blades, #22, for #4 handles
-Trays, plastic
~Part I: Separating Cell Structures
1.) Place an uncooked egg into a beaker of white vinegar. Label and cover it with plastic wrap. Leave it for 24 to 48 hours. While the shell dissolves, proceed to Part 2.
2.) Gently pick up the egg with a slotted spoon and rinse it in water to remove the vinegar after the 24 to 48 hour mark.
3.) Feel the outer membranes of the egg. Place the egg in a beaker of 5% NaCl solution for 24 hours to verify that the water can enter and leave the cell easily.
4.) After the 24 hours, gently rinse off the egg with water. Then place it into a beaker of distilled water for 24 hours to see what changes.
5.) Gently slice the egg's membranes open to let the egg white drip though the slots of the spoon into a 100-mL beaker. Then try to get all egg white into the beaker without damaging the yolk.
6.) Place the yolk of the egg into another breaker, with the egg membranes removed.
~Part II: Testing Standard Solutions
-Control so/ns +/-
-Know what is in standards
*Standard:
1.) Glucose 2.) Starch 3.) Gelatin 4.) Oil |
*Micromolecule:
Carbohydrates Carbohydrates Protein Fat |
*Indicator:
Benedict Iodine Biuret (NaOH + CuSO-1) Sudan IV |
*Description Positive Control Test: Results:
1.) 2.) 3.) 4.) |
*Description Negative Control Test Results:
|
*Monosaccharide Indicator Standard Test:
1.) Test Glucose: Mix 2mL of 2% glucose, using the pipet, with 2mL of Benedict's into a glass testing tube (flick bottom carefully of the tube for more mix). When done mixing, place the tube into a beaker with hot, boiling water and record the color change, including the time for each color change that appears.
2.) Test Water: Mix 2mL of deionized water with 2mL of Benedict's into a new glass testing tube. Put the tube into the breaker with hot, boiling water. As before with the glucose, record the changes in colors and the time between each change.
*Starch Indicator Standard Test:
1.) Test Starch: Before mixing, mix the Starch (in it's closed-tube). Now, mix the well-mixed Starch with 0.25 mL of Lugol's iodine. Lastly, you only record the color change the moment of mixing. DON'T HEAT IT!!!
2.) Test Water: Mix 2mL of deionized water with 0.25 mL of Lugol's iodine. DON'T HEAT THIS EITHER!!! Just record the color change.
*Protein Indicator Standard Test:
1.) Test Protein: 2mL of Gelatin in a test tube. Then add 0.5 mL of 10% NaOH (Sodium Hydroxide) to tube and gently mix with Gelatin. Lastly, add 0.25 mL of 5% Cu2SO4 (Copper Sulfate) to tube and mix all together. Record the color change after around 30 seconds.
*Lipid Indicator Standard Test:
1.) Test Lipids: Place a drop of Oil on a paper bag. Then let the bag sit there for around 10 minutes. Lastly, hold the paper bag and light it on fire. Record how much light passes though the Oil.
2.) Test Water: Place a drop of water on a new paper bag. Then let the bag sit there for 20 minutes. Lastly, hold the paper bag and light it. Record the light's process.
~Part III: Molecular Composition of Egg Components
1.) Conduct each indicator test in Part II, but, instead, substitute each egg component to be tested. DON'T ADD ANY OF THE STAND SOLUTIONS!!!
2.) Record all the results of each test onto another data table. Include the numbers taken from the results and a brief description of each color change in each result.
~Data/Results:
*Part I:
1.) CaCO(3)(5) + CH(3)COOH ---> H(2)O + CO(2) + H(2)CO(3) = Vinegar mixed with Calcium (egg shell)
2.) H --> L
NaCl --> into egg
H(2)O --> outside egg
3.) NaCl --> outside egg } Bigger, Bloated
H(2)O --> inside egg } Bigger, Bloated
*Part II & Part III:
1.) Test Glucose: Mix 2mL of 2% glucose, using the pipet, with 2mL of Benedict's into a glass testing tube (flick bottom carefully of the tube for more mix). When done mixing, place the tube into a beaker with hot, boiling water and record the color change, including the time for each color change that appears.
2.) Test Water: Mix 2mL of deionized water with 2mL of Benedict's into a new glass testing tube. Put the tube into the breaker with hot, boiling water. As before with the glucose, record the changes in colors and the time between each change.
*Starch Indicator Standard Test:
1.) Test Starch: Before mixing, mix the Starch (in it's closed-tube). Now, mix the well-mixed Starch with 0.25 mL of Lugol's iodine. Lastly, you only record the color change the moment of mixing. DON'T HEAT IT!!!
2.) Test Water: Mix 2mL of deionized water with 0.25 mL of Lugol's iodine. DON'T HEAT THIS EITHER!!! Just record the color change.
*Protein Indicator Standard Test:
1.) Test Protein: 2mL of Gelatin in a test tube. Then add 0.5 mL of 10% NaOH (Sodium Hydroxide) to tube and gently mix with Gelatin. Lastly, add 0.25 mL of 5% Cu2SO4 (Copper Sulfate) to tube and mix all together. Record the color change after around 30 seconds.
*Lipid Indicator Standard Test:
1.) Test Lipids: Place a drop of Oil on a paper bag. Then let the bag sit there for around 10 minutes. Lastly, hold the paper bag and light it on fire. Record how much light passes though the Oil.
2.) Test Water: Place a drop of water on a new paper bag. Then let the bag sit there for 20 minutes. Lastly, hold the paper bag and light it. Record the light's process.
~Part III: Molecular Composition of Egg Components
1.) Conduct each indicator test in Part II, but, instead, substitute each egg component to be tested. DON'T ADD ANY OF THE STAND SOLUTIONS!!!
2.) Record all the results of each test onto another data table. Include the numbers taken from the results and a brief description of each color change in each result.
~Data/Results:
*Part I:
1.) CaCO(3)(5) + CH(3)COOH ---> H(2)O + CO(2) + H(2)CO(3) = Vinegar mixed with Calcium (egg shell)
2.) H --> L
NaCl --> into egg
H(2)O --> outside egg
3.) NaCl --> outside egg } Bigger, Bloated
H(2)O --> inside egg } Bigger, Bloated
*Part II & Part III:
~Reflection:
1.) What did you like/ find interesting?
2.) How did you & your partner collaborate? (Collaboration rubric)
3.) What would you do differently next time?
~Data Analysis/Conclusion:
1.) I liked how we were able to test with different materials using new tools. I didn't do much of that at all in school before this class, so it's nice to learn how to use new, simple equipment like the Pipets and combining materials I never heard of into pretty colors, seeing how they reacted. Pretty cool. I also thought it was interesting how the eggs changed in the end of the Eggs-periment. Okay, I am seriously done with the puns (FOR NOW >:-D).
2.) I collaborated with my partners by doing the little things, like getting equipment for the process and getting paper towels to clean up. I also did writing so that if we split up to do our own thing with the experiment, we would return to each other and reflect on our data/result similarities and differences. It was cool doing it with my friends too, but as much as I like doing projects with my friends, I would like to do it with new people. Learn how to work with others I don't know.
3.) Next time I do and project with who ever, I need to be more out there. What I mean by that is try harder than I do. Summer just ended and I still need to get into the don't just do enough to get by attitude. Do more work and MORE EFFORT! I had a hard time with that my freshmen year, but now I am more mature and have thought about it more. Anyway, in conclusion, I enjoyed this project, working with my friends, and learning new experiences. I can't wait for what's in store next!
1.) What did you like/ find interesting?
2.) How did you & your partner collaborate? (Collaboration rubric)
3.) What would you do differently next time?
~Data Analysis/Conclusion:
1.) I liked how we were able to test with different materials using new tools. I didn't do much of that at all in school before this class, so it's nice to learn how to use new, simple equipment like the Pipets and combining materials I never heard of into pretty colors, seeing how they reacted. Pretty cool. I also thought it was interesting how the eggs changed in the end of the Eggs-periment. Okay, I am seriously done with the puns (FOR NOW >:-D).
2.) I collaborated with my partners by doing the little things, like getting equipment for the process and getting paper towels to clean up. I also did writing so that if we split up to do our own thing with the experiment, we would return to each other and reflect on our data/result similarities and differences. It was cool doing it with my friends too, but as much as I like doing projects with my friends, I would like to do it with new people. Learn how to work with others I don't know.
3.) Next time I do and project with who ever, I need to be more out there. What I mean by that is try harder than I do. Summer just ended and I still need to get into the don't just do enough to get by attitude. Do more work and MORE EFFORT! I had a hard time with that my freshmen year, but now I am more mature and have thought about it more. Anyway, in conclusion, I enjoyed this project, working with my friends, and learning new experiences. I can't wait for what's in store next!